Strategy to build a complete catalogue of human proteins could put China in a leading position

First the genome; now the proteome. China has already established a leading position in DNA sequencing through the work of the BGI in Shenzhen (formerly the Beijing Genomics Institute), which has been generating eye-catching genomics discoveries for the past few years (see Nature 464, 22–24; 2010). This week, Chinese researchers are set to announce initiatives that could put them at the forefront of international efforts to catalogue and characterize all proteins in the human body. Inspired by the Human Genome Project, protein researchers worldwide have been seeking funds to build a full catalogue of human proteins — a proteome — which, they say, will explain how the information encoded in our genomes can give rise to normal and diseased states.

his January, the Human Proteome Organization (HUPO) in Montreal, Canada, took the first steps towards an international effort. It set up the Human Proteome Project (HPP) working group to coordinate researchers who are characterizing the products of the 21,000 or so protein-coding genes in the human genome (see Nature 452, 920–921; 2008). But funding agencies worldwide have yet to show full support for the scheme, which is likely to carry a multi billion-dollar price tag. There is also uncertainty over the scope of the project — each human protein can be modified in an as-yet-unspecified number of ways — and over how well data from different labs can be compared (A. W. Bell et al. Nature Methods 6, 423–430; 2009).

However, this week, Chinese researchers travelled to HUPO’s annual congress in Sydney, Australia, to announce plans that defy such scepticism. The Beijing Proteome Research Center, directed by biochemist He Fuchu, has received average grants of roughly 200 million renminbi (US$30 million) per year as one of several institutes involved in HUPO’s Human Liver Proteome Project (seeNature 425, 441; 2003). That budget, He says, is soon set to double as the initiative — now renamed the Chinese Human Proteome Project — expands to cover proteins in the blood, brain, lungs, skin and other organs. The Chinese government also plans to invest 1.2 billion renminbi in a national laboratory called the Pilot Hub of Encyclopedical proteomIX (PHOENIX), which has been several years in the planning.

“The investment is impressive and unprecedented,” says Ruedi Aebersold, a proteomics expert at the Swiss Federal Institute of Technology in Zurich and a member of the HPP working group. “The launch of a large project in China would give the whole HPP project a significant boost. “The PHOENIX project will include a doubling of facilities for nuclear magnetic resonance imaging at Peking University, already the largest facility for structure determination in China but heavily oversubscribed. Tsinghua University’s electron microscope facility will be expanded, and there will be more capacity for protein-structure studies at the Institute of Biophysics of the Chinese Academy of Sciences in Beijing.

But the core of the project, and half the budget, will go to He’s new mass spectroscopy centre, to be built near the Beijing Proteome Research Center. The cash will buy 20 new mass spectrometers, bringing the total number in the centre to 29. They will be integrated with a robotic pipeline for high-throughput processing of proteins, including bioinformatic analysis. It will be the “first laboratory in the world to reach that level of integration”, says He.

China’s human proteome project will build on the success of He’s liver project, which he says produced the largest human organ proteome to date, encompassing nearly 7,000 proteins (A. Sun et al. J. Proteome Res. 9, 50–58; 2010). At the Sydney meeting, He plans to discuss unpublished work that maps protein–protein interactions and their localization. “It’s a real, comprehensive demonstration for other organs,” he says.

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